Free tool · mites per 100 bees

Alcohol wash mite count calculator

Mites ÷ bees × 100, read against the Honey Bee Health Coalition threshold for the colony's phase.

½ cup lightly packed ≈ 300 bees; fewer than 300 is not an accurate sample (HBHC). Some extension protocols use 400.

Not sure watches from 1 % and acts at 2 %. If the colony is dormant or building up in spring, pick that phase — its threshold is 1 %.

— % Type the mites you counted Wash about 300 bees (½ cup) from a brood frame to get a number.

The math is the same as the Bee Community app's, and the thresholds come from the same edition of the HBHC guide. Thresholds vary by region — consult your state apiary inspector or extension office.

The address bar keeps your numbers, so you can copy the link to share a result.

HBHC thresholds by colony phase

The Honey Bee Health Coalition sets one treatment threshold per colony phase. The calculator compares your percent with the phase you pick; "Not sure" is the band the app uses when it has no phase for a hive: watch from 1 %, act at 2 %. Pick the phase if you know it, because Dormant and Population increase act at 1 %.

Thresholds: Honey Bee Health Coalition, Tools for Varroa Management, 9th ed., June 2026 — honeybeehealthcoalition.org. "Watch from" is Bee Community's own band.
Colony phaseAct atWatch from
Dormant1 %0.5 %
Population increase1 %0.5 %
Peak population2 %1.5 %
Population decrease2 %1.5 %
Not sure (phase unknown)2 %1 %

Edition 9, dated 2026-06-11. Unit: mites per 100 adult bees (percent of a ~300-bee wash).

The "watch from" column is Bee Community's own warning band, 0.5 percentage points below each threshold, so a count that is creeping up gets a second look before it crosses the line. It is not an HBHC category. After a treatment the app looks for a count below 2 %.

How the number is computed

Percent = mites ÷ bees × 100. That is mites per 100 adult bees, the unit the HBHC guide uses for its thresholds. With a 300-bee sample the shortcut is mites ÷ 3: 12 mites in 300 bees is 12 ÷ 300 × 100 = 4 %.

The calculator, like the app, divides by the bee count you enter, never by a fixed 3, so a 200- or 400-bee sample still reads right.

Mites in a 300-bee sample → percent, with the verdict for each phase
Verdicts use the HBHC thresholds above; "watch" is Bee Community's own band just below a threshold.
MitesPer 100 beesDormant / Increase (act at 1 %)Peak / Decrease (act at 2 %)
00.0 %belowbelow
10.3 %belowbelow
20.7 %watchbelow
31.0 %at or abovebelow
41.3 %at or abovebelow
51.7 %at or abovewatch
62.0 %at or aboveat or above
72.3 %at or aboveat or above
82.7 %at or aboveat or above
93.0 %at or aboveat or above
103.3 %at or aboveat or above
113.7 %at or aboveat or above
124.0 %at or aboveat or above
134.3 %at or aboveat or above
144.7 %at or aboveat or above
155.0 %at or aboveat or above
165.3 %at or aboveat or above
175.7 %at or aboveat or above
186.0 %at or aboveat or above
196.3 %at or aboveat or above
206.7 %at or aboveat or above
217.0 %at or aboveat or above
227.3 %at or aboveat or above
237.7 %at or aboveat or above
248.0 %at or aboveat or above
258.3 %at or aboveat or above
268.7 %at or aboveat or above
279.0 %at or aboveat or above
289.3 %at or aboveat or above
299.7 %at or aboveat or above
3010.0 %at or aboveat or above

Which wash method the number is for

An alcohol wash is the HBHC's preferred method; a soap wash counts the same way, and the calculator treats both alike. A sugar roll returns the bees alive but is the less reliable count (HBHC). A sticky board measures natural mite drop over days, not mites per 100 bees; it is monitoring only and does not belong in this calculator.

Take about half a cup of bees, roughly 300, from one to three combs in the brood nest, making sure the queen is not among them. Those are nurse bees, the ones that carry the mites you want to count. Fewer than 300 bees is not an accurate sample; some extension protocols use 400, and the calculator accepts any count you enter. If there is no brood, sample from a frame of pollen.

The wash itself, step by step, is in the protocols the app cites:

When to sample

Wash about once a month from the start of population increase, and at least four times between peak population and population decrease. Wash before a treatment so you have a baseline, then again 14 to 21 days after it ends, looking for a count below 2 %. If the count is still at or above the threshold, the guide's advice is another registered product from a different chemical group — read that label too.

Under ten colonies, sample every one of them; the app defaults to that.

Thresholds vary by region

Thresholds vary by region — consult your state apiary inspector or extension office. The calculator uses the HBHC numbers above; the state and university variants below are listed so you recognize them when you see them, not as an alternative setting.

Other published variants, for reference only

Questions beekeepers ask

What does the percent mean?

Mites per 100 adult bees. Count the mites that drop out of the wash, divide by the number of bees in the sample, and multiply by 100. Twelve mites in 300 bees is 4 %. The calculator divides by the bee count you enter, never by a fixed 3.

Why 300 bees, and why half a cup?

A level half cup of lightly packed bees is about 300 bees. That is the sample the Honey Bee Health Coalition guide is built around, and the guide says a smaller sample is not accurate. Take the bees from one to three brood-nest combs, and find the queen before you shake. Some extension protocols use 400 bees; the calculator works with whatever count you enter.

Alcohol wash or sugar roll?

Alcohol or soap wash is the method the HBHC guide prefers: it gives the more accurate count, and the bees in the sample do not survive it. A sugar roll returns the bees alive but is the less reliable count (HBHC). Whichever you choose, use it every time so the trend in your log means something.

What if I have fewer than 300 bees?

Enter the count you have; the calculator divides by your number. A sample under 300 bees is not accurate (HBHC), so read the result as a rough check and wash a full half cup next time.

What does the watch result mean?

It is Bee Community's own warning band, not an HBHC category: a short band just under the threshold for the phase you picked, printed with the result. In the app that hive's status turns amber with the prompt to log a wash, so the next count comes sooner than your monthly check and you have looked at which registered products fit your temperatures and supers before it crosses the line.

Why do thresholds differ by phase and by region?

The same count means something different at different points in the colony's year, so the HBHC guide sets its thresholds by colony phase — dormant, population increase, peak population, population decrease — rather than by calendar month, and it says the numbers vary by region. Several extension services publish their own variants; the footnote on this page lists the ones on file, for reference only. When in doubt, ask your state apiary inspector or extension office which threshold they use.

The count is above the threshold. What now?

That is the guide's signal to act, not a diagnosis. Pick a registered product that fits your daytime temperatures and whether honey supers are on, read the whole label before you open the package — the label is the law — and wash again after the treatment ends to confirm the count came down; the result above gives the recheck window when a count is over the line. Two failed treatments in a season is a reason to call your state apiary inspector.

How often should I wash?

About once a month through the active season, starting when the colony begins to build in spring, and at least four times from peak population through the late-summer decline. Wash before a treatment so you have a baseline, and again after it ends to see whether it worked. The app turns that cadence into reminders from your own log.

Does a sticky board count?

No. A sticky board counts mites that fall onto a board over a period of days; it is not a count per 100 bees, so it does not map onto these thresholds. The app records it as monitoring only. Use a wash for the number you compare against the threshold.

Where do the numbers come from?

The thresholds are the ones published by the Honey Bee Health Coalition in Tools for Varroa Management, 9th edition; the edition date is printed under the table. The guide is licensed CC BY-NC-ND 4.0, so this page states the numbers as facts in its own words and copies none of the guide's text, tables or figures. The watch band is Bee Community's own addition. Bee Community is not affiliated with or endorsed by the Coalition.

Bee Community

Take it to the bee yard

Log each wash and the chart shows the trend against the threshold, with a reminder when the projection says wash again.

Bee Community is a free Android app for the bee yard: a logbook that works offline, reminders built from your own records and your region's season, a mite-wash calculator with the Honey Bee Health Coalition thresholds, a mite trend chart, and a treatment picker driven by EPA labels. Free for two active hives with no ads; Premium and Commercial add hives and tools, and nothing you record is ever locked.

Get Bee Community on Google Play

Android only. Weather and the season calendar are US-only for now. Beekeepers 18 and over. Nothing in the app is veterinary, regulatory or professional advice; the product label is the law, and your state apiary inspector is the person to call.

Questions or support: support@mybeecommunity.com

Nothing on this page is veterinary, regulatory, legal or professional advice. The product label is the law, and your state apiary inspector is the person to call. Photos, weather data and varroa guidance come from public-domain, Creative Commons and extension sources and are credited on the app's Credits screen.